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ID 19948
Eprint ID
19948
フルテキストURL
タイトル(別表記)
Fluorometric determination of ammonia in river water by flow injection analysis
著者
三笠 博司 岡山大学
本水 昌二 岡山大学
桐栄 恭二 岡山大学
抄録
Flow injection analysis(FIA) was examined for the fluorometric determination of ammonia in river water. Ammonia reacted with ο-phthalaldehyde(OPA) in the presence of 2-mercaptoethanol(ME) to form a fluorescent substance at pH 9.5. The reagent solution containing 10(-2) M OPA and 10(-3) M ME and the carrier fluid(distilled water) were propelled by a double plunger pump at a rate of 1.2 ml/min. The 40μl sample solution, injected into the carrier stream, was mixed with the reagent solution in a Teflon tubing (3 m, 0.5 mm i.d.) and led to a flow cell(18 μl). Fluorescence excited at λ(ex)=350 nm was measured at λ(em)=486 nm. Ions present commonly in river waters did not interfere with the determination of ammonia. An anion exchange column installed just behind a sample injection valve in the flow system was effective in eliminating interferences with amino acids. Using the proposed FIA system, trace amounts of ammonia(3150 ppb as nitrogen) in river water were determined in the rate of 40 samples per h.
キーワード
flow injection analysis of ammonia
reaction of ammonia with ο-phthalaldehyde and 2-mercaptoethanol
ammonia-nitrogen in waters by fluorophotometry
発行日
1985-08-05
出版物タイトル
分析化学
出版物タイトル(別表記)
Bunseki Kagaku
34巻
8号
出版者
日本分析化学会
出版者(別表記)
The Japan Society for Analytical Chemistry
開始ページ
518
終了ページ
521
ISSN
0525-1931
NCID
AN00222633
資料タイプ
学術雑誌論文
オフィシャル URL
http://www.journalarchive.jst.go.jp/japanese/jnlabstract_ja.php?cdjournal=bunsekikagaku1952&cdvol=34&noissue=8&startpage=518http://www.journalarchive.jst.go.jp/japanese/jnlabstract_ja.php?cdjournal=bunsekikagaku1952&cdvol=34&noissue=8&startpage=518
言語
Japanese
著作権者
The Japan Society for Analytical Chemistry
論文のバージョン
publisher
査読
有り