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ID 32414
JaLCDOI
フルテキストURL
著者
Matsui, Hideki Kagawa Medical School Kaken ID publons researchmap
Kurosaki, Tomohiro Okayama University
Tokuda, Masaaki Okayama University
Hatase, Osamu Kagawa Medical School
抄録

2-Mercaptoethanol increases the optical density of assay solutions at wavelengths between 280 to 400 nm, and therefore interferes with the measurement of protein concentration by the microbiuret method. Protein concentration can be determined in the presence of 2-mercaptoethanol up to 6 mM by modification of the method as follows: after the precipitation of protein by trichloroacetic acid in the presence of deoxycholate, the precipitate is resolubilized with NaOH solution. Dithiothreitol interfered with the protein determinations could by made in the presence of 4 mM of dithiothreitol with the modified microbiuret method. This modified method is time-saving and more reliable than other methods for protein determination, such as Lowry's method, in the presence of sulfhydryl reagents.

キーワード
microbiuret method
sulfhydryl reagent
protein determination
Amo Type
Article
発行日
1983-04
出版物タイトル
Acta Medica Okayama
37巻
2号
出版者
Okayama University Medical School
開始ページ
125
終了ページ
129
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT