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ID 30465
JaLCDOI
フルテキストURL
著者
Nakamura, Mitsuo Kagawa Medical School
Itano, Toshifumi Kagawa Medical School
Yamaguchi, Fuminori Kagawa Medical School
Mizobuchi, Masayuki Kagawa Medical School
Tokuda, Masaaki Kagawa Medical School
Matsui, Hideki Kagawa Medical School Kaken ID publons researchmap
Etoh, Siji Kagawa Medical School
Hosokawa, Kiyoshi Kagawa Medical School
Ohmoto, Takashi Kagawa Medical School
Hatase, Osamu Kagawa Medical School
抄録

Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system.

キーワード
protein
peptide
microdialysis
extracellular space
probe
Amo Type
Article
発行日
1990-02
出版物タイトル
Acta Medica Okayama
44巻
1号
出版者
Okayama University Medical School
開始ページ
1
終了ページ
8
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT