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ID 16836
Eprint ID
16836
フルテキストURL
Thumnail 94_667.pdf 206 KB
タイトル(別表記)
Studies of the Donaggio Reaction and reaction of small amounts of urianry protein. Part V. Comparison of modified Meulemans method and modified Iwata's method for determining specific difference in the concentration of urinary protein before and after preservation.
著者
松井 義典 岡山大学医学部公衆衛生学教室
抄録
The benzethonium chloride method of Iwata for the turbiditometric determination of minute amounts of protein reacted in alkali condition was improved for application to the measurement of urinary protein before and after physical loading. 1) In order to determine minute amounts of protein based on Iwata's method, an improved method was devised. A 0.2ml of urine was added to 3.9ml of a mixture of 33.85mol/l of EDTA 4Na and 0.5mol/l of NaOH. 2) The correlation coefficient of both modified Meulemans method and modified Iwata's one was R=0.96 and the regression equation was y=1.33x+0.17 where x was protein concentration obtained by modified Iwata's method using urine of students before Judo and basketball exercise. The correlation coefficient of both methods was R=0.98 and the regression equation was y=0.91x+0.27 using urine of students agter Judo and basketball exercise. The value obtained by both methods nearl coincided with each other. 3) Turbidity produced by modified Iwata's method was more stable after addition of reagents than that by modified Meulemans method. 4) A minute amount of urinary protein could be preserved and determined by modified Iwata's method up to 4days under freezing at -20℃.
キーワード
Meulemans変法(改良法)
岩田氏変法(改良法)
尿微量蛋白
発行日
1982-08-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
94巻
7-8号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
667
終了ページ
672
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/94/7-8/94_7-8_667/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma