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ID 13849
Eprint ID
13849
フルテキストURL
Thumnail 106_1063.pdf 3.38 MB
タイトル(別表記)
Strongly negatively charged extracellular matrix in the mouse central nervous system
著者
村上 宅郎 岡山大学医学部第二解剖学教室
抄録
Neurons of the retrosplenial cortex, hippocampal subiculum, pontine nuclei, intracerebellar nuclei and some other nuclei in the mouse demonstrated a marked surface coat which was formed three or four weeks after birth. This coat was stained doubly with cationic iron colloid (pH 1.0-1.5) and aldehyde fuchsin or with alcian blue and aldehyde fuchsin. Digestion with hyaluronidase eliminated all the cationic iron colloid, aldehyde fuchsin and alcian blue stainings of the surface coat. Successive digestion with chondroitinase ABC, heparitinase and keratanase erased the cationic iron colloid and alcian blue stainings of the surface coat, but did not interfere with the aldehyde fuchsin staining of this coat. Electron microscopy of ultrathin sections revealed that the iron particles were preferentially deposited in the perineuronal tissue spaces. These findings indicate that the surface coat consists of sulfated proteoglycans, which form an extracellular matrix in the perineuronal tissue spaces and as a charge barrier assure stable signal transmission at the axosomatic synapes with inhibitory nature. It was further observed that the neurons with such surface coats in the retrosplenial cortex and hippocampal subiculum were labeled with lectin Vicia villosa agglutinin.
キーワード
Central nervous system
neurons
strongly negatively charged extracellular matrix
sulfated proteoglycans
備考
原著
発行日
1994-10
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
106巻
9-10号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
1063
終了ページ
1071
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/106/9-10/106_9-10_1063/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma