岡山医学会 Acta Medica Okayama 0030-1558 69 8 1957 鉤虫症貧血に於ける血清鉄,銅量並びに臓器銅の態度 2169 2173 EN Kazuo Kitazima Tamotsu Sogawa Takakazu Naito Kenwo Asano Seiken Honda Akira Suizu The authors studied the iron and copper metabolisms in dogs and patients with hookworm anemia, and obtained the following results. 1) In hookworm anemia, serum iron was low in a parallel correlation with the severity of anemia as other iron deficiency anemias. Hookworm anemia, however, was different from other iron deficiency anemias in that it was accompanied by the reduction of serum copper in severe cases. Thus, Fe/Cu was also lower. 2) In the treatment of hookworm with vermicide, serum iron and copper together with Fe/Cu were restored first and afterwards followed the recovery of anemia. 3) The amount of tissue copper of animals increased in the cases of dog with hookworm anemia and in the cases of rabbit being intravenously injected with serum of dog with hookworm anemia. The increase was obvious in the liver and spleen. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 5-1 1959 特発性栓球減少性紫斑病の本態に関する臨床的並びに実験的研究 第3編 骨髄巨核球機能に及ぼす実験的栓球減少症海猽脾の影響に就いて 2283 2292 EN Seiken Honda In an attempt to study the relationship between the megakaryocyte function and the spleen in experimental thrombocytopenia, the author performed the tissue culture of the bone marrow and the spleen of normal guinea pigs and also the bone-barrow tissue culture of normal guine pigs side by side along with the spleen of the guinea pigs injected with anti-platelet serum on the same slide glass, each by a simple culture method; and observed the number and functions of megakaryocytes appearing in the growth area and the wandering velocity of neutrophils. 1. In the case of the bone-marrow tissue of normal guinea pigs cultured side by side with normal spleen on the same slide, megakaryocytes appering in the growth area was somewhat less in number and their functions were also lower than in the case of the bone-marrow tissue cultuse alone. However, there was no significant difference in the wandering velocity of neutrophils. 2. When the spleen of normal guinea pigs given anti-platelet serum was cultured side by side with bone marrow of normal guinea pigs, no significant difference in number of megakaryocytes appearing could be recognized as compared with the case with the normal spleen, but the functions were markedly decreased. The wandering velocity of neutrophils in both of these cultures was Identically the same. From these findings it has been found that in the spleen of the guinea pigs injected with anti-platelet serum there exists a factor which acts directly on megakaryocytes of the normal guinea pigs as to inhibit their platelet production. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 5-1 1959 特発性栓球減少性紫斑病の本態に関する臨床的並びに実験的研究 第2編 骨髄巨核球機能に及ぼす実験的栓球減少症海猽血清の影響に就いて 2271 2281 EN Seiken Honda With a view to study the causative factor of the experimental thrombocytopenia induced in guinea pigs by injecting anti-platelet serum into guinea pigs, the author performed the bone-marrow tissue culture of normal guinea pigs in the serum of guinea pigs with this experimental thrombocytopenia by means of the simple method of bone-marrow tissue culture, and observed the number and functions of megakaryocytes as well as the relative tissue growth rate and the wandering velocity of neutrophils. The number of megakaryocytes appearing in the tissue growth area showed no significant difference from that of the bone-marrow tissue culture in the serum of normal guinea pigs, the control, but the megakaryocyte function was markedly diminished as compared with the control, especially after 24 hours' culture no such megakaryocytes as showing the separation of platelets could at all be observed and also a marked degenerative picture was observed. The relative tissue growth rate was somewhat decreased, and the wandering velocity of neutrophils showed no significant difference from that of the control. From these findings it has become clear that in the serum of guinea pigs with experimental thrombocytopenia there exists a factor acting directly on megakarxocytes as to suppress the separation of platelets. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 5-1 1959 特発性栓球減少性紫斑病の本態に関する臨床的並びに実験的研究 第1編 特発性栓球減少性紫斑病患者血清の健康人骨髄巨核球機能に及ぼす影響 2261 2270 EN Seiken Honda With the purpose to pursue the platelet reducing factor in the serum of idiopathic thrombocytopenic purpura, the author performed the bone-marrow tissue culture of the normal persons and the patients with this disease by the simple method of bone-marrow tissue culture; and also observed number of megakaryocytes, their functions (motility and separation of platelets) in the tissue growth area, relative tissue growthrate, and the wandering velocity of neutrophils by means of the bone-marrow tissue culture of normal persons in the serum of patient with this disease. 1. In the bone-marrow tissue culture of this disease number of megakaryocytes appearing in the tissue growth area was normal in one case, and greater than the normal in two cases; but the megakaryocyte funcion was on the whole lowered. The relative growth rate and the wandering velocity of neutrophils showed no significant differences from those of the control. 2. In the case of the bone-marrow tissue culture of normal persons in the serum of this disease there was no significant difference in the number of megakryocytes appearing in the growth area from that of the control, but the megakaryocyte function was markedly diminished and also a marked degeneration was recognized. However, the relative growth rate and the wandering velocity of neutrophils showed no significant differences from those of the control. From these findings it is clear that there exists some factor in the serum of the patient with thrombocytopenic purpura, which acts directly on megakarycoytes so as to inhibit the thrombocyte production. No potential conflict of interest relevant to this article was reported.