このエントリーをはてなブックマークに追加
ID 16844
Eprint ID
16844
フルテキストURL
Thumnail 94_693.pdf 1.72 MB
タイトル(別表記)
Studies on morphology and function of mast cells and basophils Part I. Immunoglobulin receptors on mast cells
著者
岸本 卓巳 岡山大学医学部第二内科
抄録
Granules of mast cells contain chemical mediators, histamine and SRS-A, and degranulation of the cell is induced by immunological reaction. To identify a relation between immunological reaction mediated by IgE or IgG and surface ultrastructures of mast cells, a probe was constructed for immunoglobulin receptors on the cell surface by covalently coupling antigen to latex particles (0.455±0.010μm in diameter). Histamine release from mast cells and morphological aspects were examined using a technique for immune-scanning electron microscopy, when antigen was incubated with cells which were passively sensitized with antiserum. The following results were obtained: 1) IgE receptors were detected by the binding of numerous latex particles on the surface of the mast cell, in contrast with control experiments for passive sensitization with or without heat-inactivated anti-serum. Mast cells which showed ridge-and ruffle-dominant type or raspberry type, were ultrastructurally recognized in the immunological reaction, though control cells were of the microvilli-dominant type. 2) IgG-mediated reaction was recognized on the mast cell by marked release of histamine from cells and by a low degree of staining with toluidine blue. Surface ultrastructure of mast cells was also changed as those of the IgE-mediated reaction. Moreover, degranulation of mast cells was observed at the site of patch or cap formation of the receptor. In conclusion, these findings indicate that rat mast cells have both IgE and IgG receptors. Moreover, mast cells are activated and are induced to release these granules by the immunoglobulin-mediated reaction.
キーワード
mast cell
IgE receptor
IgG receptor
immune-SEM
histamine release
発行日
1982-08-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
94巻
7-8号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
693
終了ページ
706
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/94/7-8/94_7-8_693/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma