start-ver=1.4
cd-journal=joma
no-vol=
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start-page=
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dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=20070323
dt-online=
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en-subject=
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en-title=EBウイルス関連皮膚疾患における痂皮を用いた非侵襲的EBウイルス潜伏感染細胞の証明
kn-title=A novel, noninvasive diagnostic probe for hydroa vacciniforme and related disorders　Detection of latency-associated Epstein-Barr virus transcripts in the crusts
en-subtitle=
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en-abstract=
kn-abstract=OBJECTIVE: To establish a new diagnostic method for Epstein-Barr virus (EBV)-associated cutaneous disorders. DESIGN: Skin biopsy is usually required to confirm the latent EBV infections in cutaneous lesions of EBV-associated NK/T-cell lymphoproliferative disorders, including hydroa vacciniforme (HV) and hypersensitivity to mosquito bites (HMB). We have devised a novel, noninvasive method to detect EBV-encoded small RNA (EBER), BamHI A rightward transcripts (BARTs) in the skin crusts and scales of such patients. PATIENTS: Six patients with EBV-associated cutaneous lesions were enrolled in the present study, including three patients with HV, one with HV-like eruptions and chronic active EBV infection, and two with EBV-associated cutaneous lymphoma. MAIN OUTCOME MEASURES: RNA was extracted from the crusts obtained from the cutaneous lesions by forceps, converted to cDNA, and processed for polymerase chain reaction (PCR) amplification with a specific set of primers. The PCR products were assayed by a DNA sequencer. RESULTS: Intact RNAs were successfully extracted from the crusts as well as control materials. EBER1 and BARTs RNAs were detected in all 7 crusts, and in 6 of 7 crusts of EBV-associated cutaneous diseases, respectively. One of 23 crusts from non EBV-associated diseases was positive for EBER1 RNA. The sensitivity and specificity of our assay for latent EBV infection were 100% and 95.8% for EBER1 RNA, and 85.7% and 100% for BARTs mRNA, respectively. The correct DNA sequence for EBER1 and BARTs was confirmed in the PCR products by a direct sequencing method. CONCLUSIONS: Our procedure may be of use as a biomarker for EBV-associated cutaneous lesions, including HV, HMB, and NK/T-cell lymphomas.
en-copyright=
kn-copyright=

en-aut-name=YamamotoTakenobu
en-aut-sei=Yamamoto
en-aut-mei=Takenobu
kn-aut-name=山本剛伸
kn-aut-sei=山本
kn-aut-mei=剛伸
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=EB virus
kn-keyword=EB virus
en-keyword=Hydroa vacciniforme
kn-keyword=Hydroa vacciniforme
en-keyword=Noninvasive
kn-keyword=Noninvasive
en-keyword=EBER
kn-keyword=EBER
en-keyword=Crust
kn-keyword=Crust
END