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ID 17062
Eprint ID
17062
フルテキストURL
Thumnail 92_597.pdf 894 KB
タイトル(別表記)
Study on early evaluation of mixed lymphocyte culture test
著者
山本 泰三 岡山大学第一外科教室
抄録
In the clinic of renal transplantation the difference of histocompatibility matching is significant in judging the prognosis of renal transplantation. MLC test is one of the histocompatibility tests indispensable to select donor-recipient combination, but generally it takes as long as 4-6 days, and it is not easy to use as routine for the practice of urgent renal transplantation. In this study, for the purpose of clinical application of cadaver renal transplantation on the basis of protein synthesis rate with (3)H-leucine pulse label method, this method was used to conduct technical basic experiment for rapid MLC test. First in establishing the condition of culture, Eagle's MEM was used which contains no leucine in the culture, and human serum was not added. The maximum number of reacting lymphocytes in MLC test was 1×10(6)/0.2ml/well. At 24 hours after commencement of culture % SPS (stimulation of protein synthesis) became maximum, and it was decided as time of judgement. There was correlation between rapid and standard MLC tests. % SPS of 10 cases of living donor transplantation was 11.8±16.7, % SPS of 10 cases of cadaver donor transplantation 97.6±31.5, and there was a significant difference (p<0.001). About 9 cases of cadaver kidney transplantation, low HLA matching grade showed high % SPS and a tendency of bad renal function after transplantation. In the cases whose renal function was lost within 3 months after transplantation % SPS tended to be higher than in the cases whose transplanted kidney survived longer. Although there are many factors which influence the prognosis of renal transplantation, it has been suggested that at least such a combination should be selected as % SPS below 80 and HLA matching above 2 matched to maintain good renal function for a long time. From the above, this rapid MLC test has been recognized retrospectively useful to test histocompatibility matching of urgent cadaver renal transplantation.
キーワード
リンパ球混合培養
PHA
主要組織適合性抗原
死体腎移植
発行日
1980-06-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
92巻
5-6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
597
終了ページ
612
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/92/5-6/92_5-6_597/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma