岡山医学会 Acta Medica Okayama 0030-1558 71 7-1 1959 家兎皮下組織内における血色素の分解に関する研究 第3編 家兎皮下組織内に牛血色素液及び家兎抗牛血色素を注入した際の血色素heme及びglobinの変化に就て 3809 3812 EN Masao Yamamoto By injectiug the bovine hemoglobin (previously mixed with anti-hemoglobin serum and left stanbing for two hours) into the subcutaneous tissue on the inner side of the rabbit femur, the author studied changes of the globin serologically and also pursued histochemically the course taken by the decomposition of the heme. The following are the results. 1. The globin degenerated by anti-hemoglobin serum returns to the normalcy gradually after the injection into the tissue. 2. As the degeneration of the globin recovers, the heme is broken up from hemosiderin to bilirubin and then to bilifuscin, bnt no processes such as the liberation of heme accompanying the degeneration of the globin and subsequent decomposition of it can be recognized. 3. The globin is liberated as the decomposition of the heme proceeds and it is held in the tissue for a long time, but the decomposed product of the heme are absorbed rather quickly. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 7-1 1959 家兎皮下組織内における血色素の分解に関する研究 第2編 家兎皮下組織内に牛血液及び家兎抗牛血色素血清を注入した際の血色素heme及びglobinの変化に就て 3805 3808 EN Masao Yamamoto After mixing bovine blood with anti-bovine hemoglobin serum of rabbit, the mixture is injected into the subcutaneous tissue on the inner side of the rabbit femur, immediately, 2 hours later, and 5 hours later, and the author studied serologically changes of the globin at each site of the injection as well as well as carried on the histochemical observations on the decomposition of the heme. The results are described in the following. 1. The injected blood cells are destroyed in the tissue, and at the same time the injected anti-serum, existing there intact, acts so as to bring about the begeneration of the globin. The degree of the degeneration is not affected by the conditions affecting blood cells and serum. 2. The degeneration of the globin thus induced is again brought back to the normalcy by the action of the tissue. 3. The hoemglobin decomposition in the tissue is suppressed when globin is degenerated, and it proceeds after the recovery of the degeneration. This condition is evident outside the tissue than inside and it is not distinct within histiocytes. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 7-1 1959 家兎皮下組織内における血色素の分解に関する研究 第1編 家兎皮下組織内に牛血液を注入した際の血色素heme及びglobinの変化に就て 3799 3804 EN Masao Yamamoto By injecting bovine blood into the subcutaneous tissue on the inner side of the rabbit femur the author studied histochmeically the Decomposition of hemoglobin-heme as well as pursued serologically changes of hemoglobin-globin; and obtained the following results. 1. When bovine blood is injected subcutaneously, the hemoglobin decomposition takes place relatively quickly outside the histiocytes. Namely, on the second day after the injection the formation of hemosiderin, on the third day of bilirubin, and on the fifth day of hematoidin cristals, on the ninthday of bilifuscin can be recognized outside the cells; and the number of these is increased on the 14th day. On the other hand, within histiocytes are formed hemosiderin granules on the fifth day, hematoidin crystals on the seventh day, and hematoidin crystals are increased in number on the 14th day. 2. The precipitation of globin in the tissue is somewhat slow on the first day of the injection, but from the second day to the 15th day the precipitation presents the value of 32, 000-fold, and this maximum has been maintained throughout the period observation. 3. Therfore, the degeneration of globin observable at the process of the hemoglobin destruction is extremely trivial yet persistent, and this fact corroborates the theory advocated by K. Kajiro et al. 4. From the fact that hemosiderin is formed from hemoglobin, and then the hematoidin (bilirubin) or billifuscin is fomed and that the antigenic value of globin in the tissue is unchanged even at the stage when hematoma has almost completely disappeared, it is believed that globin is decomposed in the course of bilirubin formation from hemoglobin and is kept in the tissue for a long period of time. Therfore, the theory that indirect bilirubin is bilirubin-globin seems to be not appropriate. No potential conflict of interest relevant to this article was reported.