このエントリーをはてなブックマークに追加
ID 57788
フルテキストURL
著者
Toba, Shinsuke Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Minato, Yusuke Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID publons
Kondo, Yuma Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Hoshikawa, Kanami Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Minagawa, Shu
Komaki, Shiho Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kumagai, Takanori Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Matoba, Yasuyuki Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Morita, Daichi Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Ogawa, Wakano Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID
Gotoh, Naomasa Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University
Tsuchiya, Tomofusa Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kuroda, Teruo Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
抄録
We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in Serratia marcescens. We identified eight putative RND efflux system genes in the S. marcescens Db10 genome that included the previously characterized systems, sdeXY, sdeAB, and sdeCDE. Six out of the eight genes conferred multidrug resistance on KAM32, a drug hypersensitive strain of Escherichia coil. Five out of the eight genes conferred resistance to benzalkonium, suggesting the importance of RND efflux systems in biocide resistance in S. marcescens. The energy-dependent efflux activities of five of the pumps were examined using a rhodamine 6G efflux assay. When expressed in the toiC-deficient strain of E. coil, KAM43, none of the genes conferred resistance on E. coil. When hasF, encoding the S. marcescens ToIC ortholog, was expressed in KAM43, all of the genes conferred resistance on E. coil, suggesting that HasF is a major outer membrane protein that is used by all RND efflux systems in this organism. We constructed a sdeXY deletion mutant from a derivative strain of the clinically isolated multidrug-resistant S. marcescens strain and found that the sdeXY deletion mutant was sensitive to a broad spectrum of antimicrobial agents.
発行日
2019-3-19
出版物タイトル
Scientific Reports
9巻
出版者
Nature Publishing Group
開始ページ
4854
ISSN
2045-2322
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© The Author(s) 2019
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1038/s41598-019-41237-7
ライセンス
https://creativecommons.org/licenses/by/4.0/
助成機関名
日本学術振興会
助成番号
JP24590080