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ID 40725
フルテキストURL
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タイトル(別表記)
Rapid diagnosis of early Mycoplasma pneumoniae infection by indirect immunofluorescence with monoclonal antibody
著者
寺坂 薫 岡山大学医学部細菌学教室
抄録
Although serological methods or culture methods of Mycoplasma pneumoniae (Mp) from throat washings are used for diagnosis of Mp infection, the patient is often discharged from the hospital before definitive results can be obtained. Therefore we have developed a rapid detection from patients' throat smears by immunofluorescence with anti-rabbit IgG. Antibody specificity to Mp and the difference of titers between each lot have been a problem, because of polyclonal antibody which is prepared by immunizing a rabbit. Two hybridoma cell lines producing monoclonal antibodies to Mp were obtained by fusion of spleen cells from BALB/c mice immunized by Mp antigen with myeloma cells (X63-Ag8-6. 5. 3.). The high specificity to Mp of these monoclonal antibodies produced by the hybridomas was affirmed ELISA, immunofluorescence, and Western blot. A few cross-reactions were recognized by ELISA between Mp antigen and other Mycoplasma antigens. No cross-reactivity was found by indirect immunofluorescence. Throat smears from 4 patients suffered from Mp infection were examined by immunofluorescence with monoclonal antibody. Specific immunofluorescence was seen in all smears. Both granular and diffuse types of fluorescence were seen in smears from case 1 and 2. Only diffuse type was found in smears from case 3 and 4. Monoclonal antibodies can be obtained at any time and amount. The method may be useful and convenient for the rapid diagnosis of Mycoplasmal pneumonia.
キーワード
Mycoplasma pneumoniae
迅速診断法
モノクロナール抗体
間接蛍光抗体法
発行日
1989-02
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
101巻
1-2号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
189
終了ページ
199
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/101/1-2/101_1-2_189/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma