このエントリーをはてなブックマークに追加
ID 13845
フルテキストURL
著者
Kunihiko Seno 岡山大学
Kazuki Matsumura 岡山大学
大島 光子 岡山大学
本水 昌二 岡山大学
抄録
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium. A simple and fast detection system was developed using the reaction with pyridine and ethylenediamine in acidic aqueous solution and spectrophotometric flow injection analysis. The absorbances were measured at 400 nm and the reaction was accelerated at 40˚C. The calibration graph showed good linearity from 0 to 10% of EDC·HCl solutions: the regression equation was y = 3.15 × 104x (y, peak area; x, % concentration of EDC·HCl). The RSD was under 1.0%. Sample throughput was 15 h–1. This method was applied to monitoring the EDC·HCl concentration that remained after the anhydration of phthalic acid in water, esterification of acetic acid in methanol or dehydration condensation of malonic acid and ethylenediamine in water.
発行日
2008-04-10
出版物タイトル
Analytical Sciences
24巻
4号
出版者
日本分析化学会
開始ページ
505
終了ページ
508
ISSN
0910-6340
NCID
AA10500785
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
日本分析化学会
論文のバージョン
publisher
DOI