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ID 11720
Eprint ID
11720
フルテキストURL
Thumnail K003313.pdf 110 KB
タイトル(別表記)
白血病細胞におけるHLAクラスIの発現欠失・低下についての検討
著者
増田 浩三 岡山大学
抄録
Loss or down-regulation of human leukocyte antigen (HLA) class I expression has been demonstrated in a variety of solid tumors. To date, such altered HLA expression has not been studied extensively in freshly isolated leukemic blasts. If it occurs, leukemic cells could escape T-cell surveillance as a consequence. Genotypes of nine leukemic cell lines were determined using a polymerase chain reaction for HLA classes I and II. Cells were also examined for HLA beta2-microglobulin, and allele-specific HLA protein expression using flow cytometry. Next, 44 samples of freshly isolated leukemic blasts from 43 patients with malignant hematological diseases were examined for allele-specific HLA expression using flow cytometry. Microsatellite analysis was performed to determine heterozygosity in the HLA region on chromosome 6. Genotype analysis for HLA class I together with microsatellite analysis demonstrated loss of HLA haplotype in HL-60 cells. No loss of HLA haplotype was observed in 44 samples of freshly isolated leukemic blasts. As reported previously, flow cytometric analysis rarely demonstrated loss or down-regulation of HLA expression at initial diagnosis (3/39; 7.7%); however, this was evident in two of five cases in relapse (40.0%), which contrasts with previous reports. In one patient with acute leukemia, HLA-A2 cell surface expression was present at initial diagnosis, lost at relapse, and completely restored after 48 h of culture in the presence of interferon-gamma. These results suggest loss of allele-specific HLA expression may be involved in the pathogenesis of relapse in patients with leukemia. The findings should be valuable in designing new strategies for clinical immunotherapy.
キーワード
REVERSE-TRANSCRIPTASE
HISTOCOMPATIBILITY LEUKOCYTE ANTIGEN
TELOMERASE
CHRONIC MYELOID-LEUKEMIA
CYTOTOXIC T-LYMPHOCYTES
TUMOR-CELL LINES
HAPLOTYPE LOSS
LARYNGEAL CARCINOMAS
GENETIC ALTERATIONS
SURFACE EXPRESSION
CERVICAL-CANCER
備考
http://dx.doi.org/10.1111/j.1349-7006.2006.00356.x
発行日
2007-03-23
出版物タイトル
資料タイプ
学位論文
学位授与番号
甲第3313号
学位授与年月日
2007-03-23
学位・専攻分野
博士(医学)
授与大学
岡山大学
オフィシャル URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17083564&dopt=Abstract
言語
Japanese
論文のバージョン
none
査読
不明