JaLCDOI 10.18926/AMO/54987
フルテキストURL 71_2_179.pdf
著者 Nosaka, Nobuyuki| Tsukahara, Kohei| Knaup, Emily| Yabuuchi, Toshihiko| Kikkawa, Tomonobu| Fujii, Yosuke| Yashiro, Masato| Yasuhara, Takao| Okada, Ayumi| Ugawa, Toyomu| Nakao, Atsunori| Tsukahara, Hirokazu| Date, Isao|
抄録 Newly published clinical practice guidelines recommend intracranial pressure (ICP) monitoring in critical care for the management of pediatric acute encephalopathy (pAE), but the utility of ICP monitoring for pAE has been poorly studied. We recently performed direct ICP monitoring for two patients. We observed that although the direct ICP monitoring had clinical benefits with less body weight gain and no vasopressor use in both cases, this monitoring technique is still invasive. Future studies should determine the utility of non-invasive ICP monitoring systems in pAE to further improve the quality of intensive-care management.
キーワード cerebral perfusion encephalopathy child intracranial pressure neurological intensive care
Amo Type Short Communication
発行日 2017-04
出版物タイトル Acta Medica Okayama
71巻
2号
出版者 Okayama University Medical School
開始ページ 179
終了ページ 180
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2017 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 28420900
著者 Nosaka, Nobuyuki| Okada, Ayumi| Tsukahara, Hirokazu|
発行日 2017-02
出版物タイトル Acta Medica Okayama
71巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/54819
JaLCDOI 10.18926/AMO/54805
フルテキストURL 70_6_435.pdf
著者 Yamashita, Miho| Hasegawa, Kosei| Higuchi, Yousuke| Miyai, Takayuki| Okada, Ayumi| Tanaka, Hiroyuki| Tsukahara, Hirokazu|
抄録 The urinary cross-linked N-terminal telopeptide of type I collagen (uNTx) levels in infantile osteogenesis imperfecta (OI) have not been well studied. Here we investigated the levels of uNTx in infants with OI and healthy infants. We collected spot urine samples from 30 infants with OI (male/female, 14/16; Sillence classification, I/II/III/IV: 15/3/6/6; age, 5.2±4.4 months) and 120 healthy infants (male/female, 75/45; age, 5.1±4.1 months) for the measurement of uNTx levels. The uNTx levels of the OI infants were significantly lower than those of the healthy infants (mean±SD, 1,363.7±530.1 vs. 2,622.2±1,202.6 nmol BCE/mmol Cr; p<0.001). The uNTx levels of the infants with type I OI were significantly lower than those of the age-matched healthy infants, although an overlap was observed between the 2 groups. Among the 1-month-old infants, the uNTx levels of the infants with types I, III or IV OI were significantly lower than those of the healthy infants, without overlap (1,622.5±235.8 vs. 3,781.0±1,027.1 nmol BCE/mmol Cr; p<0.001). These results indicate that uNTx levels are significantly lower in infants with OI than in healthy infants, and they suggest that uNTx might be useful as a reference for diagnosing OI.
キーワード bone resorption marker bone turnover bone mass
Amo Type Original Article
発行日 2016-12
出版物タイトル Acta Medica Okayama
70巻
6号
出版者 Okayama University Medical School
開始ページ 435
終了ページ 439
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2016 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 28003667
JaLCDOI 10.18926/AMO/54500
フルテキストURL 70_4_255.pdf
著者 Nosaka, Nobuyuki| Fujiwara, Takeo| Knaup, Emily| Okada, Ayumi| Tsukahara, Hirokazu|
抄録 Estimation methods for pediatric weight have not been evaluated for Japanese children. This study aimed to assess the accuracy of mothersʼ reports of their childrenʼs weight in Japan. We also evaluated potential alternatives to the estimation of weight, including the Broselow tape (BT), Advanced Pediatric Life Support (APLS), and Parkʼs formulae. We prospectively collected cross-sectional data on a convenience sample of 237 children aged less than 10 years who presented to a general pediatric outpatient clinic with their mothers. Each weight estimation method was evaluated using Bland-Altman plots and by calculating the proportion within 10 and 20 of the measured weight. Mothersʼ reports of weight were the most accurate method, with 94.9 within 10 of the measured weight, the lowest mean difference (0.27kg), and the shortest 95 limit of agreement (-1.4 to 1.9kg). The BT was the most reliable alternative, followed by APLS and Parkʼs formulae. Mothersʼ reports of their children ʼs weight are more accurate than other weight estimation methods. When no report of a childʼs weight by the mother is available, BT is the best alternative. When an aged-based formula is the only option, the APLS formula is preferred.
キーワード body weight child estimation techniques mothers, parents
Amo Type Original Article
発行日 2016-08
出版物タイトル Acta Medica Okayama
70巻
4号
出版者 Okayama University Medical School
開始ページ 255
終了ページ 259
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2016 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 27549669
Web of Sience KeyUT 000384748600004
タイトル(別表記) Basic and clinical research regarding vascular endothelial function
フルテキストURL 127_187.pdf
著者 塚原 宏一|
キーワード アルギニン代謝 一酸化窒素 ガス生物学 血管内皮学 酸化ストレス
備考 総説(Reviews)
出版物タイトル 岡山医学会雑誌
発行日 2015-12-01
127巻
3号
出版者 岡山医学会
出版者(別表記) Okayama Medical Association
開始ページ 187
終了ページ 195
ISSN 0030-1558
NCID AN00032489
資料タイプ 学術雑誌論文
言語 Japanese
著作権者 Copyright (c) 2015 岡山医学会
論文のバージョン publisher
査読 有り
DOI 10.4044/joma.127.187
NAID 130005116819
Sort Key 4
Eprints Journal Name joma
参考文献 1) Furchgott RF, Zawadzki JV : The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature (1980) 288, 373-376. 2) Palmer RM, Ferrige AG, Moncada S : Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature (1987) 327, 524-526. 3) Culotta E, Koshland DE Jr : NO news is good news. Science (1992) 258, 1862-1865. 4) 塚原宏一 : 窒素 (N) と酸素 (O) の化学・生物学. 日臨高気圧酸素潜水医会誌 (2010) 7, 1-8. 5) 塚原宏一, 吉本順子 : 一酸化窒素, アルギニン代謝と酸化ストレス. 別冊「医学のあゆみ」レドックスUPDATEストレス制御の臨床医学・健康科学 (2015), 103-111. 6) Cooke JP : ADMA : Its role in vascular disease. Vasc Med (2005) 10, S11-17. 7) Tsukahara H, Nagasaka H, Tokuriki S, Mayumi M : Coupling of the citrulline recycling to endothelial NO production. Mol Genet Metab (2010) 99, 438. 8) Morris CR, Gladwin MT, Kato GJ : Nitric oxide and arginine dysregulation : A novel pathway to pulmonary hypertension in hemolytic disorders. Curr Mol Med (2008) 8, 620-632. 9) Pernow J, Jung C : Arginase as a potential target in the treatment of cardiovascular disease : Reversal of arginine steal? Cardiovasc Res (2013) 98, 334-343. 10) Kubo M, Ogino K : Analytical procedures for nitrative/nitrosative stress ; in Oxidative Stress in Applied Basic Research and Clinical Practice-Pediatric Disorders, Tsukahara H, Kaneko K (eds), Springer, Berlin (2014) pp149-158. 11) Tsukahara H, Ishida T, Mayumi M : Gas-phase oxidation and disproportionation of nitric oxide. Methods Enzymol (2002) 359, 168-179. 12) Nishio M, Tsukahara H, Hiraoka M, Sudo M, Kigoshi S, Muramatsu I : Calcium channel current in cultured rat mesangial cells. Mol Pharmacol (1993) 43, 96-99. 13) Tsukahara H, Gordienko DV, Goligorsky MS : Continuous monitoring of nitric oxide release from human umbilical vein endothelial cells. Biochem Biophys Res Commun (1993) 193, 722-729. 14) Tsukahara H, Gordienko DV, Tonshoff B, Gelato MC, Goligorsky MS : Direct demonstration of insulin-like growth factor-I-induced nitric oxide production by endothelial cells. Kidney Int (1994) 45, 598-604. 15) Goligorsky MS, Budzikowski AS, Tsukahara H, Noiri E : Co-operation between endothelin and nitric oxide in promoting endothelial cell migration and angiogenesis. Clin Exp Pharmacol Physiol (1999) 26, 269-271. 16) Tsukahara H, Ende H, Magazine HI, Bahou WF, Goligorsky MS : Molecular and functional characterization of the non-isopeptide-selective ETB receptor in endothelial cells. Receptor coupling to nitric oxide synthase. J Biol Chem (1994) 269, 21778-21785. 17) Goligorsky MS, Tsukahara H, Magazine H, Andersen TT, Malik AB, Bahou WF : Termination of endothelin signaling : role of nitric oxide. J Cell Physiol (1994) 158, 485-494. 18) Gordienko DV, Tsukahara H : Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar artery and human umbilical vein. Pflugers Arch (1994) 428, 91-93. 19) Tsukahara H, Miura M, Tsuchida S, Hata I, Hata K, Yamamoto K, Ishii Y, Muramatsu I, Sudo M : Effect of nitric oxide synthase inhibitors on bone metabolism in growing rats. Am J Physiol (1996) 270, E840-845. 20) Tsukahara H, Hori C, Hiraoka M, Yamamoto K, Ishii Y, Mayumi M : Endothelin subtype A receptor antagonist induces osteopenia in growing rats. Metabolism (1988) 47, 1403-1407. 21) Tsukahara H, Hiraoka M, Hori C, Hata I, Okada T, Gejyo F, Sudo M : Chronic erythropoietin treatment enhances endogenous nitric oxide production in rats. Scand J Clin Lab Invest (1997) 57, 487-493. 22) Tsukahara H, Hori C, Tsuchida S, Hiraoka M, Fujisawa K, Mayumi M : Role of endothelin in erythropoietin-induced hypertension in rats. Nephron (1998) 79, 499-500. 23) Tsukahara H, Imura T, Tsuchida S, Nunose M, Hori C, Hiraoka M, Gejyo F, Sudo M : Renal functional measurements in young rats with chronic inhibition of nitric oxide synthase. Acta Paediatr Jpn (1996) 38, 614-618. 24) Tsukahara H, Hiraoka M, Kobata R, Hata I, Ohshima Y, Jiang MZ, Noiri E, Mayumi M : Increased oxidative stress in rats with chronic nitric oxide depletion : Measurement of urinary 8-hydroxy-2'-deoxyguanosine excretion. Redox Rep (2000) 5, 23-28. 25) 長坂博範, 塚原宏一 : 先天性代謝疾患と一酸化窒素, 酸化ストレス. 発達腎研会誌 (2010) 18, 29-36. 26) Nagasaka H, Tsukahara H, Yorifuji T, Miida T, Murayama K, Tsuruoka T, Takatani T, Kanazawa M, Kobayashi K, Okano Y, Takayanagi M : Evaluation of endogenous nitric oxide synthesis in congenital urea cycle enzyme defects. Metabolism (2009) 58, 278-282. 27) Nagasaka H, Yorifuji T, Egawa H, Inui A, Fujisawa T, Komatsu H, Tsukahara H, Uemoto S, Inomata Y : Characteristics of NO cycle coupling with urea cycle in non-hyperammonemic carriers of ornithine transcarbamylase deficiency. Mol Genet Metab (2013) 109, 251-254. 28) Nagasaka H, Okano Y, Tsukahara H, Shigematsu Y, Momoi T, Yorifuji J, Miida T, Ohura T, Kobayashi K, Saheki T, Hirano K, Takayanagi M, et al. : Sustaining hypercitrullinemia, hypercholesterolemia and augmented oxidative stress in Japanese children with aspartate/glutamate carrier isoform 2-citrin-deficiency even during the silent period. Mol Genet Metab (2009) 97, 21-26. 29) Nagasaka H, Okano Y, Aizawa M, Miida T, Yorifuji T, Tajima G, Sakura N, Takatani T, Sanayama Y, Sugamoto K, Mayumi M, Kobayashi K, et al. : Altered metabolisms of mediators controlling vascular function and enhanced oxidative stress in asymptomatic children with congenital portosystemic venous shunt. Metabolism (2010) 59, 107-113. 30) Nakatsukasa Y, Tsukahara H, Tabuchi K, Tabuchi M, Magami T, Yamada M, Fujii Y, Yashiro M, Tsuge M, Morishima T : Thioredoxin-1 and oxidative stress status in pregnant women at early third trimester of pregnancy : relation to maternal and neonatal characteristics. J Clin Biochem Nutr (2013) 52, 27-31. 31) 塚原宏一, 中務陽子, 山田睦子, 重安良恵, 吉本順子, 喜多村哲朗, 長坂博範, 森島恒雄 : Early third trimesterの妊婦における血清中NOx, ADMA(内因性NO合成酵素阻害因子)および酸化ストレスマーカー. 日小児高血圧研会誌 (2013) 10, 8-15. 32) Tsukahara H, Ishida T, Mayumi M : Gas-phase oxidation of nitric oxide : Chemical kinetics and rate constant. Nitric Oxide (1999) 3, 191-198. 33) Tsukahara H, Ishida T, Todoroki Y, Mayumi M : Gas-phase disproportionation of nitric oxide at elevated pressures. Free Radic Res (2003) 37, 171-177. 34) Tsukahara H, Todoroki Y, Hiraoka M, Mayumi M : N2O anesthesia may exacerbate hyperhomocysteinemia and endothelial dysfunction in patients with renal impairment. Kidney (2004) 65, 338. 35) Tsukahara H : Redox modulatory factors of human breast milk ; in Handbook of Dietary and Nutritional Aspects of Human Breast Milk, Zibadi S, Watson RR, Preedy VR (eds), Wageningen Academic Publishers, Netherlands (2013) pp599-614. 36) Servillo L, Giovane A, Cautela D, Castaldo D, Balestrieri ML : The methylarginines NMMA, ADMA, and SDMA are ubiquitous constituents of the main vegetables of human nutrition. Nitric Oxide (2013) 30, 43-48.
JaLCDOI 10.18926/AMO/53674
フルテキストURL 69_5_279.pdf
著者 Saito, Yukie| Fujii, Yousuke| Yashiro, Masato| Tsuge, Mitsuru| Nosaka, Nobuyuki| Yamashita, Nobuko| Yamada, Mutsuko| Tsukahara, Hirokazu| Morishima, Tsuneo|
抄録 Lung hyperpermeability affects the development of acute respiratory distress syndrome (ARDS), but therapeutic strategies for the control of microvascular permeability have not been established. We examined the effects of edaravone, dexamethasone, and N-monomethyl-L-arginine (L-NMMA) on permeability changes in human pulmonary microvascular endothelial cells (PMVEC) under a hypercytokinemic state. Human PMVEC were seeded in a Boyden chamber. After monolayer confluence was achieved, the culture media were replaced respectively by culture media containing edaravone, dexamethasone, and L-NMMA. After 24-h incubation, the monolayer was stimulated with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Fluorescein-labeled dextran was added. Then the trans-human PMVEC leak was measured. Expressions of vascular endothelial-cadherin (VE-cadherin) and zonula occludens-1 protein (ZO-1) were evaluated using real-time quantitative polymerase chain reaction and immunofluorescence microscopy. The results showed that TNF-α+IL-1β markedly increased pulmonary microvascular permeability. Pretreatment with edaravone, dexamethasone, or L-NMMA attenuated the hyperpermeability and inhibited the cytokine-induced reduction of VE-cadherin expression on immunofluorescence staining. Edaravone and dexamethasone increased the expression of ZO-1 at both the mRNA and protein levels. Edaravone and dexamethasone inhibited the permeability changes of human PMVEC, at least partly through an enhancement of VE-cadherin. Collectively, these results suggest a potential therapeutic approach for intervention in patients with ARDS.
キーワード pulmonary microvascular endothelial cells permeability edaravone vascular endothelial-cadherin zonula occludens-1 protein
Amo Type Original Article
発行日 2015-10
出版物タイトル Acta Medica Okayama
69巻
5号
出版者 Okayama University Medical School
開始ページ 279
終了ページ 290
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2015 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 26490025
Web of Sience KeyUT 000365519600004