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ID 17390
Eprint ID
17390
フルテキストURL
Thumnail 90_841.pdf 411 KB
タイトル(別表記)
Studies on the cornin extracted from liver 2. Purification of the rat liver microsomal cornin and its chemical properties
著者
土井 昭孚 岡山大学医学部第一生理学教室
抄録
A curude cornin extracted from rat liver microsomes and the chemical properties of the purified cornin (MF1) were investigated. 1. Crude rat liver microsomal cornin (RLMC) inhibits the growth of L-929 cells. MF1 is purified about 4 fold from RLMC by gel filtration through Sephadex G-50 column. 2. The concentration of MFI that inhibits the growth of L cells to 50% is 270 μg/ml and this specific activity is the highest of the cornin fractions reported up to date. 3. The content of acidic amino acids is more than 23% but that of histidine and sulfurcontaining amino acids is very low in MFl. 4. MFl shows the maximum ultraviolet absorption at 276nm and its main substance is a kind of protein, and its inhibitory effect on the cell growth diminishes by protease treatment. 5. MFl is a single peak both by polyacrylamide gel electrophoresis and by thin layer gel filtration and the molecular weight is calculated about 26,000. 6. MFl is separated in two bands by SDS-polyacrilamide gel electrophoresis and the molecular weight is estimated to be 11,000 and 15,000 respectively.
発行日
1978-08
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
90巻
7-8号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
841
終了ページ
849
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/90/7-8/90_7-8_841/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma