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ID 16688
Eprint ID
16688
フルテキストURL
Thumnail 93_897.pdf 592 KB
タイトル(別表記)
Studies on superoxide production of human neutrophils Part 3. In vitro and in vivo studies on the effects of antileukemic agents on the superoxide production of neutrophils
著者
厚井 文一 岡山大学医学部第2内科
抄録
Previous work, described in part 2 of this study, showed that the superoxide production of neutrophils was suppressed by induction chemotherapy. These findings suggest direct effects of antileukemic agents on intracellular bactericidal activity; however, there is little information available concerning this aspect. The superoxide of neutrophils is one of the active oxygens and it is thought to be one of the major substances related to the intracellular bactericidal activities of neutrophils. This experiment was conducted in order to evaluate the direct effects of antileukemic agents on superoxide production of human neutrophils. The following results were obtained; 1. The effects of antileukemic agents on superoxide production were examined before and after intensification chemotherapy in 14 patients with complete remission. Significant suppression of superoxide production was found 60 minutes after initiation chemotherapy; however, it recovered 24 hours after chemotherapy. 2. In the in vitro system, neutrophils were incubated with antileukemic agents for 3 minutes prior to stimulation by Concanavalin A and Cytochasin D. Among the antileukemic agents examined, Methotrexate, Vincristine and Prednisolone inhibited superoxide production with a concentration dependency. The 50 % inhibition concentrations of these agents were 3.6x10(-4)mol for Methotrexate, 3.2x10(-4)mol for Vincristine and 4.2x10(-4)mol for Prednisolone. Cytosine arabinoside, Neocartinostatine and Cyclophosphamide showed no inhibitory effects on superoxide production. 3. The inhibition of superoxide production by Methotrexate could not be reversed by Folinic acid in the medium. 4. In the cell free superoxide production system consisting of Xanthine plus Xanthine oxidase, Methotrexate and Prednisolone inhibited superoxide production with a concentration dependency. The 50% inhibition concentrations were 1.3x10(-3)mol for Methotrexate and 2.4x10(-3)mol for Prednisolone. 5. In the in vitro system, Methotrexate and Prednisolone were not only inhibitors of superoxide production but also scavengers of superoxide. These results suggest that some antileukemic agents directly inhibit intracellular bactericidal activities, resulting in the susceptibility to infection of patients with acute leukemia.
キーワード
superoxide production
neutrophilic granulocyte
antileukemic agent
acute leukemia
発行日
1981-10-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
93巻
9-10号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
897
終了ページ
907
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/93/9-10/93_9-10_897/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma