このエントリーをはてなブックマークに追加
ID 16658
Eprint ID
16658
フルテキストURL
Thumnail 93_873.pdf 611 KB
タイトル(別表記)
Studies on superoxide production of human neutrophils Part 1. Experimental studies on superoxide production of human neutrophils
著者
厚井 文一 岡山大学医学部第2内科
抄録
Patients with acute leukemia are prone to get infections. Pathological conditions such as leukocytopenia and disturbances of humoral and cellular immunity are thought to contribute to this high susceptibility to infection. Superoxide, one of the active oxygens, is thought to be related to the intracellular bactericidal activities of neutrophils, particularly in oxygen dependent systems. These findings led the author to speculate that primary and secondary disturbances in the superoxide production of neutrophils are of importance in the high susceptibility of patients with acute leukemia. In this experiment, the superoxide production of normal human neutrophils stimulated by Concanavalin A and Cytocharacin D was assayed by spectrophotometer in order to evaluate its usefulness in clinical applications. The effect of age and sex on supreoxide production was also examined. The following results were obtained: 1. The activity of superoxide production was affected by both time and temperature during cell preservation. It was also dependent on the cell concentration, temperature and pH during stimulation of the neutrophils. The optimal system for assay of the superoxide production of human neutrophils was as follow: Cells should be preserved at 0℃ within 60 minutes. During stimulation, cell concentration, temperature and pH should be ajusted to 0.2-0.5x10(6)/ml, 37℃ and 7.4, respectively. 2. There were no significant changes in superoxide production between males and females. Superoxide production in older subjects(70-89 y/o) was significantly lower than that in younger subjects(20-69 y/o)(p<0.001). The values were 4.72±1.17 nmol/min/10(6)-PMNs in the former and 6.45±1.33 nmol/min/10(6)PMNs in the latter. Assays of superoxide production of human neutrophils can be clinically used because of its simplicity and reproducibility. It was apparent that the assessment of superoxide production of neutrophils by age was necessary in clinical applications.
キーワード
superoxide production
neutrophilic granulocyte
aging neutrophil
発行日
1981-10-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
93巻
9-10号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
873
終了ページ
883
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/93/9-10/93_9-10_873/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma