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ID 55413
フルテキストURL
著者
Maeda, Megumi Department of Biofunctional Chemistry, Division of Bioscience, Okayama University Graduate School of Natural Science and Technology Kaken ID
Chen, Ying Department of Hygiene, Kawasaki Medical School
Lee, Suni Department of Hygiene, Kawasaki Medical School
Kumagai-Takei, Naoko Department of Hygiene, Kawasaki Medical School
Yoshitome, Kei Department of Hygiene, Kawasaki Medical School
Matsuzaki, Hidenori Department of Hygiene, Kawasaki Medical School
Yamamoto, Shoko Department of Hygiene, Kawasaki Medical School
Hatayama, Tamayo Department of Hygiene, Kawasaki Medical School
Ikeda, Miho Department of Hygiene, Kawasaki Medical School
Nishimura, Yasumitsu Department of Hygiene, Kawasaki Medical School
Otsuki, Takemi Department of Hygiene, Kawasaki Medical School
抄録
We have previously reported that chronic, recurrent and low-dose exposure to asbestos fibers causes a reduction in antitumor immunity. Investigation of natural killer (NK) cells using an in vitro cell line model and comprising in vitro activation using freshly isolated NK cells co-cultured with chrysotile fibers, as well as NK cells derived from asbestos-exposed patients with pleural plaque (PP) or malignant mesothelioma (MM), revealed decreased expression of NK cell activating receptors such as NKG2D, 2B4 and NKp46. An in vitro differentiation and clonal expansion model for CD8+ cytotoxic T lymphocytes (CTLs) showed reduced cytotoxicity with decreased levels of cytotoxic molecules such as granzyme B and perforin, as well as suppressed proliferation of CTLs. Additionally, analysis of T helper cells showed that surface CXCR3, chemokine receptor, and the productive potential of interferon (IFN)γ were reduced following asbestos exposure in an in vitro cell line model and in peripheral CD4+ cells of asbestos-exposed patients. Moreover, experiments revealed that asbestos exposure enhanced regulatory T cell (Treg) function. This study also focused on CXCR3 expression and the Th-17 cell fraction. Following activation with T-cell receptor and co-culture with various concentrations of chrysotile fibers using freshly isolated CD4+ surface CXCR3 positive and negative fractions, the intracellular expression of CXCR3, IFNγ and IL-17 remained unchanged when co-cultured with chrysotile. However, subsequent re-stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin resulted in enhanced IL-17 production and expression, particularly in CD4+ surface CXCR3 positive cells. These results indicated that the balance and polarization between Treg and Th-17 fractions play an important role with respect to the immunological effects of asbestos and the associated reduction in antitumor immunity.
備考
This is an article published by Spandidos Publications
This fulltext availavle in Nov 2017
発行日
2017-05-09
出版物タイトル
International Journal of Oncology
50巻
6号
出版者
Spandidos
開始ページ
2024
終了ページ
2032
ISSN
1019-6439
NCID
AA10992511
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン
publisher
PubMed ID
DOI
Web of Sience KeyUT
関連URL
https://doi.org/10.3892/ijo.2017.3991