このエントリーをはてなブックマークに追加
ID 31723
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1000 KB
著者
Araki, Shinako Okayama University
Miyagi, Yasunari Okayama Red Cross General Hospital
Kawanishi, Kunihiro Okayama University
Yamamoto, Junko Okayama University
Hongo, Atsushi Okayama University
Kodama, Junichi Okayama University
Yoshinouchi, Mitsuo Okayama University
Kudo, Takafumi Okayama University
抄録

The in vitro radiosensitizing effects of docetaxel have been reported, but the DNA damage caused by the irradiation after docetaxel exposure has not been investigated. In this study, the authors attempted to evaluate the radiosensitizing effects in terms of cell survival and DNA single-strand breaks in a human ovarian adenocarcinoma cell line (known as line BG-1) and a human cervical squamous cell carcinoma cell line (known as line SiHa). The cell lines were exposed to various concentrations of docetaxel (from 2.27 x 10(-3) to 2.27 microg/ml) to investigate the cytocidal effects by colony-formation assay. DNA single-strand breaks after exposure to 2.27 microg/ml of docetaxel for 30 min or 100 min were measured by the alkaline-elution assay. The remarkable cytotoxicity of docetaxel followed by irradiation was observed when concentrations were greater than 2.27 x 10(-2) microg/ml in both cell lines. The combination of docetaxel and irradiation appears to be supraadditive. The DNA single-strand breaks induced by the irradiation were enhanced in both cell lines (BG-1; P < 0.01, SiHa; P < 0.05). The synergistic cytocidal effect cannot be explained quantitatively only by the single-strand breaks.

キーワード
docetaxel
DNA single-strand break
radiosensitizer
Amo Type
Article
発行日
2002-02
出版物タイトル
Acta Medica Okayama
56巻
1号
出版者
Okayama University Medical School
開始ページ
13
終了ページ
18
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT