Hikichi, Yasufumi
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
Takeuchi, Shigeharu
Laboratory of Plant Pathology, Kochi Agricultural Research Center
Okumura, Ako
Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
Nasu, Yoshiko
Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
Okuno, Tetsuro
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
Suzuki, Kazumi
Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
発行日
抄録
A method for efficient inactivation Papper mild mottle virus (PMMoV) in harvested seeds of green pepper was examined based on the infectivity on the leaves of Nicotina glutinosa L.(a local host) and a reverse transcription and polymerase chain reaction (RT-PCR)-based amplification of the viral RNA. The seed homogenates from PMMoV-infected plants produced a large number of necrotic local lesion in N.glutinosa, but soaking the seeds in 10% (w/v) tri-sodium phosphate (Na3PO4) for 20 min or dry sterilization (70C,3h) effectivity eliminated the abundance of PMMoV. However, no necrotic lesion on N.glutinosa or the RT-PCR-basaed amplification was observed with seeds that has been disinfected by Na3PO4 in combination with dry sterilization.
キーワード
Capsicum annuum L.
pepper mild mottle virus (PMMoV)
RT-PCR
seed disinfection
seed transmission
ISSN
NCID