Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

Co-purification of Plasma Membrane ATPase and Phosphatidylinositol Kinase from Pea Plasma Membranes

豊田 和弘 岡山大学 ORCID Kaken ID publons researchmap
一瀬 勇規 岡山大学 ORCID Kaken ID publons researchmap
山田 哲治 岡山大学
白石 友紀 岡山大学 Kaken ID publons researchmap
抄録
The plasma membrance ATPase was partially purified by a linear glycerol density gradient centrifugation of the detergent-solubilized plasma membrance poteins and subsequent separation by a size-exclusion column chromatogrphy. A purified ATPase preparation is shown to contain a 97.6kDa protein that was cross-reacted with an antibody raised against mung bean H+-ATPase. The preparation also exhibited the phosphorylation of exogenous phosphatidylionsitol(PI) when supplized with [γ-32P]ATP. These results indicate that one form plasma membrance ATPase is co-purified with PI kinase.
キーワード
Lipid kinase
Mycosphaerella
plasma membrance ATPase
pea(Pisum sativum L.)
suppressor
ISSN
0474-0254
NCID
AN00033029