Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

モノクロナール抗体結合制癌剤に関する研究 第1編 抗HLAモノクロナール抗体結合制癌抗生剤(ブレオマイシン,マイトマイシンC,マクロモマイシン)の作製とその生物活性

眞鍋 雄一 岡山大学医学部第2内科
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抄録
Bleomycin (BLM) and mitomycin C (MMC), potent anticancer agents, were linked covalently to murine monoclonal anti-HLA IgG(1) antibody (H-1) with the use of dextran T-40. Macromomycin (MCR) was coupled to H-1 in the presence of carbodiimide. H-1 alone did not inhihit cell growth. BLM-(H-1) and MMC-(H-1) conjugates showed stronger cytotoxicity to an HLA-bearing cell line than BLM and MMC, respectively. MCR-(H-1) and free MCR showed the same cytotoxicity to HLA-bearing cells, while the conjugate was less toxic than free MCR against cells lacking HLA. These results indicate that BLM-(H-1), MMC-(H-1) and MCR-(H-1) exert antibody-targeting cytotoxicity in vitro.
キーワード
抗HLAモノクロナール抗体
bleomycin
mitomycin C
macromomycin
ISSN
0030-1558
NCID
AN00032489