Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

自己免疫性甲状腺疾患における甲状腺組織障害機序の研究 第2編 甲状腺上皮細胞を用いたADCCの検討

三浦 洋 岡山大学医学部第三内科学教室
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抄録
In the presence of decomplemented sera from 13 out of 14 patients with Hashimoto's disease and 6 out of 7 patients with Graves' disease, normal human leucocytes caused significant lysis of cultured thyroid cells, as estimated by the release of (51)Cr. Cytolysis was seen to proceed linearly during the first 9 hours, but ceased after that. Cytolysis was enhanced by the addition of complement at as low as the concentration which did not induce cytotoxic factor mediated lysis. A study of different effector cells mediating ADCC indicated that mononuclear and non-adherent cells were cytotoxic to cultured thyroid cells, but adherent cells were not effective in this system. The percentage of cytotoxicity correlated with the anti-microsomal antibody(MCHA) titer (r=0.63, p<0.01), but not with the anti-thyroglobulin antibody (TGHA) titer. Inhibition of ADCC by aggregated IgG suggested that immune complexes in the sera of patients with thyroid disorders might inhibit cell destruction by an ADCC mechanism.
キーワード
甲状腺上皮細胞
ADCC
ISSN
0030-1558
NCID
AN00032489