Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.

成人T細胞性白血病(Adult T-cell leukemia)に関する研究 第2編 白血病細胞のTdTとMonoclonal抗体により検出されるT細胞分化抗原の検討

十川 重次郎 岡山大学医学部第2内科教室
95_701.pdf 378 KB
TdT and surface T-cell antigens of leukemic cells from 14 patients with ATL were analysed with a TdT immunofluorescent assay kit(BRL) and monoclonal antibodies (Becton-Dickinson, Leu-1, Leu-2a and Leu-3a antibodies). The TdT assay kit and the monoclonal antibodies were tested to confirm their specificities using various types of cultured cell lines and fresh hematopoietic malignant cells. The reactivities of the kit and antibodies were consistent with the previously reported specificities. TdT activity in leukemic cells from 6 out of 7 ATL patients was not detected; however, cells from one patient did contain weak TdT activity. The majority of the ATL cells reacted with Leu-1 and Leu-3a antibodies but not with Leu-2a. The lack of or weak TdT activity and the expression of Leu-1 and Leu-3a reactive T-cell antigens of ATL cells indicate that ATL cells are of mature and helper/inducer T-cell origin.
Adult T-cell leukemia
Monoclonal antibody
Helper/inducer T-cell