Journal of Okayama Medical Association
Published by Okayama Medical Association

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モノクロロベンゼン中毒に関する研究 第1編 モノクロロベンゼンの尿中代謝産物(p-クロロフェニルメルカプツール酸及び4-クロロカテコールの抱合物)の高速液体クロマトグラフィーによる定量法について

嶋田 義弘 岡山大学医学部公衆衛生学教室
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抄録
The quantitative determination of major urinary metabolites of monochlorobenzene by high-performance liquid chromatography(HPLC) was studied. Urinary p-chlorophenylmercapturic acid was assayed directly and urinary monoglucuronide and ethereal sulphate conjugates of 4-chlorocatechol were assayed as 4-chlorocatechol after being hydrolyzed. The results obtained were as follows. 1. Para-chlorophenylmercapturic acid. a. Urinary p-chlorophenylmercapturic acid of rats given monochlorobenzene by subcutaneous injection was recognized as a separate peak on the HPLchromatogram against the other peaks in normal rat urine. b. A linear relationship was obtained between the quantity of p-chlorophenylmercapturic acid and its peak height on HPLchromatogram up to 5ng. 2. 4-Chlorocatechol. a. 4-Chlorocatechol in the hydrolyzate of the urine of rats given monochlorobenzene by subcutaneous injection was recognized as a separate peak on the HPLchromatogram against the other peaks of normal rat urine. b. A linear relationship was obtained between the quantity of 4-chlorocatechol and its peak height on the HPLchromatogram up to 0.3μg.
キーワード
モノクロロベンゼン
p-クロロフェニルメルカプツール酸
4-クロロカテコール体
高速液体クロマトグラフィー
ISSN
0030-1558
NCID
AN00032489