A one-way mixed lymphocyte reaction between lymphocytes from normal human donors and mitomycin C-treated cells from a human leukemic null-cell line (NALL-1) was investigated. Human leukemic T-cell and B-cell lines, an Epstein-Barr virus (EBV)-negative Japanese Burkitt's lymphoma line, and two EBV-positive normal B-cell lines were studied in parallel. Normal lymphocytes were stimulated significantly by the cultured null-and Bcells but not by the cultured T-cells. The stimulatory capacity of this null-cell line was approximately equal to that of the leukemic, lymphoma, and normal B-cell lines, and was unrelated to the presence of complement receptor, surface immunoglobulin, EBV-associated nuclear antigen, terminal deoxynucleotidyl transferase or the origin of the cell lines. These results suggest that not only leukemic B-cells but also leukemic null-cells have stimulatory determinants in mixed lymphocyte culture.
Mixed lymphocyte culture
acute lymphocytic leukemia