癌と細胞性免疫 第1編 Methylcholanthrene-誘発腫瘍の同系移植マウスの部位別リンパ組織の抗腫瘍性

Regional axillary lymph nodes, spleen and distant mesenterial lymph nodes were taken out one, two, three and four weeks after isografting methycholanthrene-induced tumor (MC-tumor) on the back under the skin of C(3)H mice and lymphocytes were prepared from these lymph nodes and spleen. These lymphocytes were then mixed with the primary cultured tumor cells of MC-tumor in the 20:1 ratio, and cultured for 24 and 48 hours in order to determine antitumor activity of the lymphocytes. As a result it has been shown that at an early stage of one week after tumor transplantation a strong antitumor activity appears only in the regional axillary lymph nodes, which weakens gradually thereafter, and as the time elapses two to three weeks, it disappears. In the spleen, a strong antitumor activity can been ovserved in the third week after tumor transplantation, but the activity disappears by the fourth week. In the distant mesenterial lymph nodes, the antitumor activity grows stronger along with lapse of time after the transplantation, which reaches its peak by the fourth week. These findings indicate that antitumor activity of lymphocytes in different sites appears earliest and strongest in the regional lymph nodes, which weakens and disappears as the cancer progresses, which an antitumor activity makes its appearance in further distant lymphatic tissues.