C(3)Hマウス乳癌におけるウイルス抗原の螢光抗体法による研究 第2編 C(3)Hマウスにおける乳癌ウイルス抗原の螢光抗体法補体法による観察

As mentioned in the author's previous study, the complement method of immunofluorescence allowed to observe clearer fluorescence of the viral antigen than by the direct and indirect method. In this study, therefore, localizaton of viral antigens of C(3)H mammary cancer was persued with the fluorescescent serum that reacted immunologically with the complement. 1. The viral antigen was observed in tumor cells and intercellular spaces and particularly rich in perivascular portions. 2. In the metastastatic lymph nodes viral antigens were observed in some tumor cells, and in the spleen of tumorbearing mice they were observed within retculum cells on rare ocasions. 3. Intracellular localization of viral antigens was spread in the peripheral portions of the cytoplasm of tumor cells, and no specific fluorescence was observed in the nucleus of tumor cells. 4. No specific fluorescence was seen in the liver, kidney, lung, bone marrow and brain of the tumor-bearing C(3)H mice and in tissues of normal mice. 5. In mice with spontaneous mammary cancer, viral antigens were encounted more frequently than in mice with transplanted tumors.