腹水癌細胞のエネルギー代謝に関する研究 第3編 腹水癌細胞における脂質過酸化反応と脂肪酸組成

The mitochondria isolated from ascites tumor cells have a specific characteristic different from those isolated from normal liver cells, i. e. they do not react with ferrous ion, GSH with GSSG and ascorbic acid, showing no increase in lipid peroxide formation and no swelling. From the viewpoint that this characteristic of tumor cell mitochondria may be due to the specific liqid. compositions of mitochondrial membrane, the author tried to analyze the components of liqids of tumor cells comparing with those of brain, liver, heart, kidney and spleen from normal animals. The following have been revealed; 1. The tumor cells are very low in peroxide formation in the presence of the reducing agents comparing to those from the normal tissues, though the grade of peroxide formation is different in each tissues and by the agents used, and is rather lower in homogenate than in intact mitochondria. 2. Chemical analysis revealed that the lipids from tumor cells is low in unsaturated fatty acid contents, especially C20-polyene contents than those from normal cells. This will be responsible for the low level in lipid peroxidation of the tumor cells. 3. On the basement of the these results discussions has been made on the lipid peroxidation of mitochondria of tumor cells and related mitochondrial swelling under the consideration of enzymic activities.

学位番号 乙第230号