This study was performed to compare the unsaponifiable fraction of lipids in the liver of the rats bearing Walker carcinosarcoma and the same tumor with the normal liver. The alumina column chromatography (alumina: containing water at 10% level) was used for the separation of carotene, vitamin A and ubiquinone. The concentrations of these substances, particuraly ubiquinone, were lower in the liver of the rats with Walker tumor and in the tumor itself than in the normal liver.
On the thin layer chromatography, the unsaponifiable fractions dissolved in petroleum benzine were separated into about 12 spots, using cyclohexane-ethyl acetate (5:1) for developmental solvent. On this development system, carotene, ubiquinone vitamin A were separated each other at Rf 0.81, 0.49 and 0.19, respectively. Carotene, however, was mixed with cholesterol ester, and vitamin A with cholesterol at the same Rf value. The concentration of the substances at all Rf values, except at Rf 0.51, were lower in the liver of the rat with Walker carcinosarcoma than in the normal liver. It was interesting that the substance at Rf 0.51, the concentration of which is higher in the liver of the transplanted rats, showed the similar absorption spectrum as that of ubiquinone and the positive Craven's test. It was suggested, therefore, that the Rf 0.51 spot probably contains some kind of quinone derivatives.