Biliverdin was obtained from cystallized bilirubin by adding hydrogen peroxide in hydrochloric acid alcohol. The biliverdin, separated in the column chromatography with an absorbent of silica gel and a developer of glacial acid, was confired by physicochemical features, absorption spectra in the ultraviolet and visible ranges. In its infrared absorption spectra, a broad absorption band ranging over 6.06 μ to 6.40 μ, other than the absorption band of carbonyl group at 5.91 μ (the C=O streching vibration of the carbonyl group (C=O)), was detected, and it was deemed probably responsible for mixture of the ionized carboxyl group instead of water of crystallization. Also the absorption band ranging over 3.80 μ to 5.00 μ. of hydrochloric acid was observed. Consequently, it was presumed that the separated, by our method, contained not only biliverdin but also hydrochloric double salt and probably mixture of acetic acid double salt. When hydrochloric acid was neutralized with caustic soda before the column chromatography, in its infrared absorption spectra, the absorption band of hydrochloric acid have disappeared, but the one of the ionized carboxyl group was still noted, presumably due to mixture of acetic double salt of biliverdin stemmed from glacial acetic acid using as a developer.