Phosphatases in serum and organs (the liver, adrenal gland, spleen, jejunum and the bone marrow) were estimated biochemically after injection of the cancer tissue extract into rabbits, and following results were obtained. 1) Following injection of cancer extract serum phosphatases were decreased, though, alkali phosphatase was slightly increased only when the toxic effect was severe. 2) In the organs of normal rabbits alkali phosphatase was larger in amount than acid phosphatase, excepting only the adrenal gland in reverse. Acid phosphatse was largest in amount in the jejunum, subsequently the spleen, adrenal gland, kidney, liver and the bone marrow. Alkali phosphatse was also largest in the jejunum, subsequently the kidney, spleen, liver, bone marrow and the adrenal gland. 3) Influences of injection of the cancer extract on the phosphatases in organs were as follows: The liver; alkali phosphatse was increased but no changes in acid phosphatse.
The adrenal gland; alkali phosphatse was increased but no changes in acid phosphatse. The spleen, no changes in the phosphatses were observed. The kidney; decreases in both phosphatases were seen. The je junum; decreases in both, especially marked in alkali phosphatase. The bone marrow; no changes were observed, though, alkali phosphatase was slightly increased when the toxic effect was severe. 4) It was surmised that an intimate relation between the liver and the phosphatase was present.