The influences of vitamin C on uric acid formation were investigated with normal male guinea pigs by the administration of 50 mg of vitamin C into seminal vesicles through vas deferens. The experiments were carried out exactly the same as stated in report 1 and results were as followed. 1. The mean uric acid concentration in blood increased significantly at 15 minutes (5 times higher than normal) and reached a peak at 30 minutes (6 times higher than normal) and then gradually decreased at 5 hours which was not still returned to normal. 2. Comparing with the continuous and interval administration, the latter experiment showed much stronger results than the former and the increasing of the total doses manifest the increase of uric acid formation but not always paralleled.