The conversion of (14)C-labelled glucose to free amino acids by homogenates of brain tissues from idiopathic epleptics, non-epileptics as well as normal and LCLA rabbits was studied. 1) The turnover of (14)C-labelled glucose to free amino acids in idiopathic epileptic brain was more disturbed than that in non-epileptic brain: to asparagine and glutamine no significant differences wee seen, but especially to gluamic acid it was slightly disturbed and to aspartic acid and γ-aminobutyric acid they were intensively disturbed. In idiopathic epileptic brain no γ-aminobutyric acid was proved. 2) In one case of focal epileptic brain, the conversion of aspartic acid was more restrained in the focus than in the contrast part. 3) In LCLA rabbit brain, the turnover of free amino acids were generally more disturbed than that by normal one. To glutamine and asparagine no significant difference was found but to glutamic acid it was slightly and to γ-aminobutyric acid it was markedly disturbed.