The specimen taken in the same way as part Ⅰ was sectioned in 20 μ slices with Sartorius freezing microtome, stretched on a cover glass and dried up. Thus the vital specimen was made. It was stained in KOELLE modification and the activity of ChE in nerve cells was histochemically investigated. 1) In idiopathic epileptics the activity of ChE in nerve cells in premotor area of the cerebral cortex showed no significant variation. 2) In all cases the activity of specific ChE was very weak. There was almost no activtity of non-specific ChE. As above mentioned, there was histochemically no difference of ChE activity between the idiopathic epileptics and the contrast in this experiment. It seems to be due to the fact that this experiment was performed for the cerebral cortex, in which ChE activity is the least.