The method of experiment of acute pancreatic necrosis in dogs was same as the described in Chapter I. The measurement was performed by the following method. Free amino acid in the pancreatic tissue was extracted by Dent's method.
Chromatography was performed by a two-dimensional method using phenol and lutidincollidine solution, and phenol and butanol-acetic acid solution. The following results were obtained. In the normal pancreatic tissue the follwing 8 free amino acids were found: cystine, aspartic acid, glutamic acid, serine, glycine, lysine, arginine and threonine. In the diseased pancreatic tissues, leucine, valine and tyrosine were found besides the above eight.