By the mix-culture of Escherichia coli O(1)O(25) by the Kauffman's classification with Salmonella typi in bouillon, the author obtained the following results: 1) Escherichia coli O(3), O(12) and O(1) were most powerfully agglutinated by anti-typhoid serum, of which the agglutination titer was 160× of the serum. 2) The bacterial antagonism of Escherichia coli to Salmonella typhi varied with the difference of types of the Coli groups, of which O(1), O(3), O(8), O(17) and O(23) were most powerfully antagonistic to Salmonella typhi. 3) Escherichia coli which was severely antagonistic to Salmonella typhi grew more rapidly than the not so one.