抗原,抗体および補体の物理化学的研究 第2篇 濾紙電気泳動法による抗体局在の研究

The procedures of filter-paper electrophresis and extraction from it, were employed to determine the distribution of antibodies. The following results were obtained: 1) Antibodies of anti-crystalline hen's egg albumin rabbit sera were found in γ-globulin fraction and slightly in β-globulin fraction. 2) Antibodies of anti-goat red blood cells rabbit sera were found in γ-globulin fraction. 3) Antibodies of anti-hen's red blood cells rabbit sera were found in γ-globulin fraction. 4) Forssman's antibody of rabbit sera was found in γ-globulin (γ(l)-globulin) fraction, and slightly in β-globulin fraction. These facts were also demonstrated by salting-out method. 5) Antibodies of anti- "bacillus coli communis" rabbit sera were found in γ-globulin and somewhat in β-globulin fraction. 6) Antibodies of anti-A and anti-B blood cells human sera were found in γ-globulin fraction.