Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

鉄ニトリロ三酢酸腹腔内投与により発生したラット腎癌からの培養細胞株の樹立とその性情

掛橋 千賀子 岡山県立大学保健福祉学部
森 將晏 岡山県立大学保健福祉学部
川端 晃幸 岡山大学医学部第一病理学教室
岡田 茂 岡山大学医学部第一病理学教室
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抄録
The characteristics of ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma by intraperitoneal administration and the effects of hormones on the tumor have been studied in vivo. However, to our knowledge there have been no reports on the characteristics of the tumor cells. In this study, we established a tumor cell line from Fe-NTA induced rat renal tumour and investigated tumor cell characteristics. The tumor cell line RKFe was established from Fe-NTA induced rat renal tumor by collagenase digestion. RKFe showed epithelial morphology in culture, and had microvilli and basement membranes under electron microscopy. RKFe cells acquired tolerance to cytotoxicity of Fe-NTA. They also grew in soft agar culture and formed tumors by the inoculation of cells into nude mice. Histologically, transplanted tumors showed poorly defferentiated adenocarcinoma with several tubular structures. A subclone of RKFe, named RKFe-C6, showed metastasis to lungs. The RKFe cell line may be useful for studying the characteristics of Fe-NTA induced rat tumor cells.
キーワード
Fe-NTA
腎癌
培養細胞株の樹立
備考
原著
ISSN
0030-1558
NCID
AN00032489