Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

ラット虚血性急性腎不全における腎内メタロチオネインの誘導

原田 圭子 岡山大学医学部麻酔・蘇生学教室
106_811.pdf 2.79 MB
発行日
1994-08
抄録
To clarify the functions of the renal metallothioneins (MTs) in rats following ischemic acute renal failure, the expression of MT mRNA and the localization of MT in rats undergoing 60 min of bilateral renal artery clamp ischemia followed by reperfusion was examined by Northern blot analysis, in situ hybridization and immunohistochemical analysis. Induction of maximum at 1 to 3 days followed by a gradual decrease. Histlogically, both MT mRNA and MT were detected in renal tubular cells intensely and specifically in the outer medulla at 1 day after release of the clamp. These findings suggest that MTs protect the tubular cells by scavenging the free radicals, which probably cause renal tubular cell damage by ischemia-reperfusion, and that MTs provide the zinc ions to zinc-requiring apo-enzymes necessary for healing and regenerating renal tubular cells.
キーワード
虚血性急性腎不全
メタロチオネイン
in situ hybridization
免疫組織化学染色
備考
原著
ISSN
0030-1558
NCID
AN00032489