Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

Biotin 標識 probe を用いた histo in situ hydridization 法による胃癌組織における癌遺伝子 mRNA の検討

田中 出 岡山大学医学部第一外科学教室
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抄録
Expression of oncogene mRNA in gastric cancer was studied by in situ hybridization. Cancer tissues were fixed in formalin and embeded in paraffin. Then, DNA-mRNA hybridization was performed by using two biotin-labeled cDNA probes (c-myc, c-Ha ras). Oncogene mRNA was intracuelularly localized, mainly in the cytoplasm, and also found in the nucleus of partial cells. Higher concentrations of oncogene mRNA vere found in advanced cancers than in early cancers, and in metastatic positive cases of the above n2 than of n0 or n1. This suggests that the expression of oncogene mRNA is related to the malignant potency of cancer.
キーワード
histo in situ hybridization 法
胃癌
癌遺伝子
mRNA
備考
原著
ISSN
0030-1558
NCID
AN00032489