There are many problems in intractable asthmatics, such as corticosteroid dependency. The clinical course is different from atopic asthma. To clarify the differences between both type of asthma, we investigated the mechanism of LAR, which has similar features to the pathogenesis of intractable asthma. We have previously reported that IgG antibody was involved with the LAR. In this study, to clarify the role of IgG subclass, we observed the density and distribution of IgG1 and IgG4 antibodies bound to the peripheral blood basophils from bronchial asthmatics using a technique of immunoscaning electron microscopy. A study revealed a relative ratio of IgG1 to IgG4 antibodies on basophils of asthmatics with Candida-provoked LAR which was higher than that of asthmatics with house dust-provoked IAR and LAR. Furthermore, in the study of immunoglobulin distribution on the basophil surface, the proportion of cap and patch formation of IgG1 antibody was higher in bronchial asthmatics with Candida-provoked LAR than in those with house dust-provoked IAR and LAR. These data suggest that IgG1 antibody may play an important role in the pathogenesis of LAR, especially Candida-provoked asthmatic response.