茨城県でダイズから分離されたアズキモザイクウイルスの諸性質

Azuki bean mosaic virus (ABMV) was isolated from Glycine max showing mild mosaic and stunt on the leaves, collected in Ibaraki-Prefecture, in July, 1989. ABMV was transmitted by sap-inoculation to 8 species systemically and 14 species locally out of 33 species in 8 families and by aphids, Aulacorthum solani and Myzus persicae in a non-persistent manner.Seed transmission was recognized in 0.8% of the seeds of soybean cv."Iwate-wase-kurome" inoculated at seeding stage. The soybean plant infected with ABMV produced mottled seeds, which were radical- or saddle-like. The virus particles were flexuous rods, bout 750 nm in length. In ultrathin virus-infected tissues, cytoplasmic inclusions containing pinwheels and scrolls were observed in the cytoplasms. In DAS-ELISA, the virus reacted strongly with antiserum to ABMV. The weight of plants and seed of two soybean cv."Okuhara 1" and "Iwate-wase-kurome" inoculated with ABMV at the seedling stage was about 40～50% less than that of healthy plants.