ID | 61263 |
フルテキストURL | |
著者 |
Hirano, Minako
Bio Photonics Laboratory, The Graduate School for the Creation of New Photonics Industries
Yamamoto, Daiki
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Asakura, Mami
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hayakawa, Tohru
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
ORCID
Kaken ID
publons
researchmap
Mise, Shintaro
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University
Matsumoto, Akinobu
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University
Ide, Toru
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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抄録 | Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, alpha-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.
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キーワード | ion channel
lipid bilayer
single-channel recording
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発行日 | 2020-12-01
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出版物タイトル |
Micromachines
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巻 | 11巻
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号 | 12号
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出版者 | MDPI
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開始ページ | 1070
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ISSN | 2072-666X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2020 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/mi11121070
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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助成機関名 |
日本学術振興会
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助成番号 | 15K13725
18K06157
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