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ID 40790
フルテキストURL
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タイトル(別表記)
Heat stability of catalase activity in the blood and liver of acatalasemic mice Part 1. Heat stabilty of catalase activity after administration of phenylhydrazine hydrochloride subcutaneously
著者
小柏 道子 岡山大学医学部公衆衛生学教室
抄録
In order to investigate the heat stability of catalase in immature and mature erythrocytes of normal, acatalasemic and heterozygous hypocatalasemic mics, phenylhydrazine hydrochloride (PHZ) was administered to these mice at a dose of 40 μg/g of body weight subcutaneously daily for 3 days Anemic blood rich in reticulocytes was obtained 2 days after final injection. Thermostability of catalase in the non-anemic and anemic bloods was compared and the following results were obtained. 1. Specific catalase activity (catalase activity/amount of hemoglobin; PU/g Hb) in anemic blood from acatalasemic mice was significantly higher than that in non-anemic blood. Specific catalase activity in anemic blood from normal mice was a little higher than that in non-anemic blood from normal mice. 2. The residual catalase activity in the hemolysate after heat treatment was compared with that from before treatment in which hemolysate containing catalase was incubated at 42, 44, 46, 48 and 50 °C for 10 minutes in a water bath. At any temperature, catalase activity in immature red blood cells of normal and acatalasemic mice was more stable with heat than that inmature red blood cells. 3. In mormal mice, the T(50) (the temperature at which 50 % of the catalase activity remained after heat treatment) in anemic normal blood was statistically higher than that in non-anemic blood. In the acatalasemic mice, the T(12.5) (the temperature at which 12.5 % of the catalase activity remained after heat treatment) in anemic blood was statistically higher than that in non-anemic blood.
キーワード
アカタラセミアマウス
フェニルヒドラジン
カタラーゼ
熱安定性
発行日
1989-12
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
101巻
11-12号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
991
終了ページ
997
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/101/11-12/101_11-12_991/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
OAI-PMH Set
岡山大学
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Sort Key
2
Eprints Journal Name
joma