ID | 34234 |
フルテキストURL | |
著者 |
Morimoto, Riyo
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Uehara, Shunsuke
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Yatsushiro, Shouki
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Juge, Narinobu
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Hua, Zhaolin
Departments of Neurology and Physiology, Graduate Programs in Neuroscience and Cell Biology, University of California San Francisco School of Medicine
Senoh, Shigenori
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Echigo, Noriko
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Hayashi, Mitsuko
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Mizoguchi, Toshihide
Institute for Oral Science, Matsumoto Dental University
Ninomiya, Tadashi
Institute for Oral Science, Matsumoto Dental University
Udagawa, Nobuyuki
Department of Biochemistry, Matsumoto Dental University
Omote, Hiroshi
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Kaken ID
researchmap
Yamamoto, Akitsugu
Department of Cell Biology, Nagahama Institute of Bioscience and Technology
Edwards, Robert H
Departments of Neurology and Physiology, Graduate Programs in Neuroscience and Cell Biology, University of California San Francisco School of Medicine
Moriyama, Yoshinori
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Kaken ID
publons
researchmap
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抄録 | Osteoclasts are involved in the catabolism of the bone matrix and eliminate the resulting degradation products through transcytosis, but the molecular mechanism and regulation of transcytosis remain poorly understood. Upon differentiation, osteoclasts express vesicular glutamate transporter 1 (VGLUT1), which is essential for vesicular storage and subsequent exocytosis of glutamate in neurons. VGLUT1 is localized in transcytotic vesicles and accumulates L-glutamate. Osteoclasts secrete L-glutamate and the bone degradation products upon stimulation with KCl or ATP in a Ca2+-dependent manner. KCl- and ATP-dependent secretion of L-glutamate was absent in osteoclasts prepared from VGLUT1-/- knockout mice. Osteoclasts express mGluR8, a class III metabotropic glutamate receptor. Its stimulation by a specific agonist inhibits secretion of L-glutamate and bone degradation products, whereas its suppression by a specific antagonist stimulates bone resorption. Finally, it was found that VGLUT1-/- mice develop osteoporosis. Thus, in bone-resorbing osteoclasts, L-glutamate and bone degradation products are secreted through transcytosis and the released L-glutamate is involved in autoregulation of transcytosis. Glutamate signaling may play an important role in the bone homeostasis.
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キーワード | osteoclast
vesicular glutamate transporter
transcytosis
bone resorption
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備考 | Digital Object Identifer:10.1038/sj.emboj.7601317
Published with permission from the copyright holder. This is the author's copy, as published in EMBO Journal, Sep 2006, Volume 25, Issue 18, Pages 4175-4186. Publisher URL:http://dx.doi.org/10.1038/sj.emboj.7601317 Direct access to Thomson Web of Science record Copyright © 2006 European Molecular Biology Organization |
発行日 | 2006-09-20
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出版物タイトル |
EMBO Journal
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巻 | 25巻
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号 | 18号
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出版者 | European Molecular Biology Organization
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開始ページ | 4175
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終了ページ | 4186
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ISSN | 0261-4189
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NCID | AA10627582
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | European Molecular Biology Organization
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論文のバージョン | author
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査読 |
有り
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DOI | |
Submission Path | biochemistry/7
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