ID | 32848 |
JaLCDOI | |
フルテキストURL | |
著者 |
Huang, Zhenyu
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Liu, Qingping
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Li, Wenzhe
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Wang, Renjun
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Wang, Dan
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Zhang, Yingbiao
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Zhang, Fan
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Chi, Yan
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Liu, Zhe
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Matsuura, Eiji
Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Kaken ID
publons
researchmap
Liu, Zibo
The No. 10 Senior High School of Dalian Economical and Technological Development Area
Zhang, Qiming
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
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抄録 | We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB. |
キーワード | 7-KC
NF-?B
apoptosis
atherosclerosis
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Amo Type | Original Article
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出版物タイトル |
Acta Medica Okayama
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発行日 | 2010-04
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巻 | 64巻
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号 | 2号
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出版者 | Okayama University Medical School
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開始ページ | 85
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終了ページ | 93
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ISSN | 0386-300X
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NCID | AA00508441
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資料タイプ |
学術雑誌論文
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言語 |
英語
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論文のバージョン | publisher
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査読 |
有り
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PubMed ID | |
Web of Science KeyUT |