Donaggio反応及び尿微量蛋白反応に関する研究 第4報 高校生の運動負荷前後の比濁法による迅速尿中微量蛋白量について

In order to measure the physical load of training of members who belong to the Judo and Basketball teams, urinary proteins before and after routine training were measured by the turbidity method (modified meulemans method) of members. The following results were obtained. 1) In members of the Basketball club, significant increase in actual urinary protein concentration was observed between noon and evening on the first day, between morning and noon, between noon and evening on the second day, between morning and noon, between morning and evening on the third day and between morning and evening on the fourth day. Significant increase in urinary protein-concentration corrected to a urine density of 1.024 was observed between noon and evening on the first day, between morning and evening on the second day, between morning and noon, between morning and evening on the third day, and between morning and evening on the fourth day. Significant increase a excretion per minute of urinary protein was observed between noon and evening on the first day, between morning and noon and between morning and evening on the third day. 2) In members of the Judo club, significant increase in actual urinary protein concentration was observed between noon and evening on the first day, between morning and noon, between morning and evening on the second day, and between morning and noon on the fourth day. Significant increase in protein-concentration corrected to a urine density of 1.024 was observed between noon and evening on the first day, between morning and evening on the second day, and between morning and noon on the fourth day. Significant increase in the excretion per minute of urinary protein was observed between noon and evening of the first day, between morning and noon on the second day, and between morning and noon on the fourth day. 3) The existence of Pre (α1)AG, (α1)AT, (α1)X, I(α)I, Zn(α2), (β2)AC, Myogl, C(3)act., Tf, (β2)III, IgA, (β2)I, Alb, and IgG of serum subfractions were recognized by immunodiffusion in an antigencontained gel layer. In particular, excretion of myoglobin was recognized. The experiments on optical density of the turbidity method of each fraction were discussed. 4) The main subjective symptoms (from questionnaires) which increased with physical loads were "Whole body getting tired", "Legs getting tired" and "Wanting to lie down" in group I in the basketball club, and "Whole body getting tired", "Legs getting tired", "Feel unsteady standing", "Becoming drowsy", "Wanting to lie down" in group I, "Feeling stiff in the shoulders", "Feeling thirsty" and "Tremor in limbs" in group III in the Judo club. 5) The preliminary care of fatigued members was discussed.