ID | 13577 |
Eprint ID | 13577
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フルテキストURL | |
タイトル(別表記) | Effect of phospholipase A(2) on proliferation of the primary cultured biliary epithelial cells from the extra-hepatic bile duct to rats using D-valine containing medium
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著者 |
小倉 薫
香川医科大学小児外科学
渡辺 泰宏
香川医科大学小児外科学
平峯 千春
香川医科大学基礎看護学
中川 利孝
香川医科大学附属実験実習機器センター
土岐 彰
香川医科大学小児外科学
徳田 雅明
香川医科大学生理学
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抄録 | We hypothesized that a high level of phospholipase A(2) (PLA(2)) in bile juice works as a growth factor of biliary epithelial cells and causes hyperplastic change of the bile duct in pancreaticobiliary maljunction. in order to examine this hypothesis, we established a primary culture system of biliary epithelial cells from the extra-hepatic bile duct of rats. We successfully excluded fibroblasts from the epithelial cells using D-valine-containing medium and the single-cell cloning method. The established cells exhibited the characteristic features of rat bile duct epithelial cells. They were stained with anti-cytokeratin antibody, a marker protein of epithelial cells, were positive with PAS stain, and possessed the architectures of microvilli, tonofilament fibers and desmosomes. The effect of PLA(2) on proliferation of these cells was then examined. We clearly demonstrated that PLA(2) at nano-molar concentration (50nM, 100nM), that is, equivalent to the level of PLA(2) in the bile juice of pancreaticobiliary maljunction cases, caused a significant enhancement of epithelial cell proliferation.
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キーワード | 膵・胆管合流異常
胆管上皮細胞
D-バリン
Phospholipase A(2)
細胞増殖
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備考 | 原著論文
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発行日 | 2003-01-31
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 114巻
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号 | 3号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 289
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終了ページ | 296
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/114/3/114_3_289/_article/-char/ja/
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関連URL | http://www.okayama-u.ac.jp/user/oma/index.html
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言語 |
日本語
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著作権者 | 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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Eprints Journal Name | joma
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